The biotype of citrus nematode (Tylenchulus semipenetrans) from Morocco was determined using differential plant hosts, namely trifoliate orange (Poncirus trifoliata), bitter orange (Citrus aurantium), grape (Vitis vinifera) and olive (Olea europaea). The study was conducted under greenhouse conditions with 5 replicates. Nematodes from regions of Gharb, Souss-Masssa, Haouz, Loukkos and Moulouya were extracted from roots and inoculated to hosts with 10.000 eggs and juveniles per plant. Reproductive factors were less than one for Poncirus trifoliate and olive for all populations. Result of differential-host experiment showed that all five T. semipenetrans populations belong to the Mediterranean biotype.

Keywords: Citrus nematode, regions, Differential host test, Mediterranean biotype.